Early Differential Expression of Galactomannan Biosynthesis Genes in ‘Makapuno’ Coconut (Cocos nucifera L.) revealed by the De Novo Assembly and Analysis of Endosperm Transcriptome

Makapuno is a coconut cultivar with a naturally overproliferating solid endosperm almost filling the nut cavity. At 6-7 months after pollination (MAP), makapuno solid endosperm is phenotypically indistinguishable from the normal type Laguna Tall while at 8-9 MAP makapuno starts to soften and thicken in contrast to the hard and compact appearance of the normal. Here, the expression profiles of 6-7 and 8-9 MAP de novo assembled RNA Sequencing (RNA-Seq) transcriptomes of normal and makapuno were analyzed, and corresponding stages were compared to determine the differentially expressed genes (DEGs).

Transcriptome Analysis of ‘Philippine Lono Tall’ Coconut (Cocos nucifera L.) Endosperm Reveals Differential Expression of Genes Involved in Oil Biosynthesis

The ‘Philippine Lono Tall’ (PLNT) is a variant of the more common ‘Philippine Laguna Tall’ (LAGT), which produces fruits with soft endosperm and reported higher fat content. To understand patterns of fatty acid (FA) and oil accumulation in LAGT and PLNT fruits, transcriptomes of 6–7 month-old endosperm samples were analyzed by RNA-Seq. Quantitative PCR was performed to analyze the differential expression of selected genes related to oil biosynthesis. Further, oil samples from the PLNT endosperm were analyzed to determine their FA composition across developmental stages.

Cytogenetics in Plant Breeding: Conservation and Evolution in the Post-Genomics Era

This seminar features the PGC hosted Balik Scientist Dr. Nomar Waminal who will be presenting his talk on “Cytogenetics in Plant Breeding: Conservation and Evolution in the Post-Genomics Era”. Dr. Waminal is currently serving as a Research Professor under the Chromosome Research Institute at Sahmyook University, Seoul, Republic of Korea. He was a Samsung Global Scholar and received his Ph.D. in Crop Science and Biotechnology from Seoul National University.

Draft Genome Sequence of Multidrug-Resistant Vibrio parahaemolyticus Strain PH698, Infecting Penaeid Shrimp in the Philippines

The emergence of multidrug-resistant bacterial strains in diverse settings has been reported globally. In the Philippine shrimp aquaculture industry, antibiotics are used for the treatment of bacterial diseases during the production cycle. We report the draft genome of Vibrio parahaemolyticus PH698, a multidrug-resistant strain isolated from a Philippine shrimp farm.

Draft Genome Sequence of Vibrio parahaemolyticus Strain PH1339, Which Causes Acute Hepatopancreatic Necrosis Disease in Shrimp in the Philippines

Acute hepatopancreatic necrosis disease (AHPND), which affects multiple shrimp species, including the economically important Penaeus monodon and Penaeus vannamei, is characterized by massive sloughing of the epithelial cells of the hepatopancreas to the point of atrophy (1).

Plant cytogenomics in the post-genomics era

The release of the first human reference genome in 2003 marked the beginning of the post-genomics era when genetics progressed beyond gene-centered analyses to a myriad of omics approaches. Genome sequencing technologies have advanced dramatically from Sanger, second/next-generation, to third-generation sequencing platforms. Also, genome assembly methods and tools have evolved contemporaneously, resulting in a dramatic drop in genome assembly cost. Consequently, more than 360 plant species’ genomes have been assembled ever since the first plant genome, Arabidopsis thaliana, was published in 2000.

“Oh, my Genes”: Lessons from Plant Genetics and Genomics for Trait Improvement

In recent years, genetics and genomics has played an important role in facilitating the genetic improvement of plants. Different tools have been developed to examine the structure, function, and properties of genes controlling complex traits. The genetic diversity of germplasm found in existing gene banks through different breeding populations such as core collections, nested association mapping panels, mutants, recombinant inbred lines, and multiparent advance generation intercross populations in germplasm banks served as sources of desirable alleles for plant breeding.