Protein, Proteomics, and Metabolomics Facility
Bridging the Gaps of Omics Research in the Philippines—PPMF aims to promote the continued improvement of Omics-based research in the Philippines and to provide proteomic and metabolomic services for research projects conducted by local researchers.
Capacity building for omics analysis
The Protein, Proteomics and Metabolomics Facility (PPMF) hosts research projects, and offer services centered on the assessment of total protein expression (proteomics) as well as total metabolite populations in test samples (metabolomics). These provide the capability to assess how genomic potentials are translated to functional traits.
Further characterization of key proteins may be done with the PPMF Suite of protein analysis services. These cover applications for the production, purification, and biophysical analysis of target protein samples.
898-18500 loc 4711
These methods aim to break down cell membranes for the release and isolation of proteins and metabolites from the specimen for further purification and analysis.
These are a selection of miscellaneous processing methods of samples performed prior to purification and analysis.
These procedures aim to purify proteins of interest from a crude protein extract. Proteins in solution (e.g. cell lysate) can be separated based on their interactions with different chromatographic columns via the ÄKTA Pure 25 L1 FPLC System.
These methods are for determining the concentrations of biomolecules in samples using colorimetric methods.
These methods are comprised of different techniques such as polyacrylamide gel electrophoresis (PAGE) for molecular weight determination, western blot analysis for detection of specific molecules, surface plasmon resonance (SPR) analysis for studying the binding kinetics of molecular interactions, Circular Dichroism – Optical Rotatory Dispersion (CD-ORD) spectroscopy for secondary structure determination.
This powerful method analyzes compounds and biomolecules by fragmenting them into ions that can be detected by the Orbitrap Fusion Tribrid high resolution mass spectrometer. Prior to mass spectrometry, sample components will be separated by reverse-phase liquid chromatography using Vanquish Horizon UHPLC for metabolite extracts or Ultimate 3000 RSLCNano nano HPLC for peptide/protein samples.
To our valued clients
Due to health and safety protocols during this pandemic, some of the offered services may not be available at all times.
Please contact us for inquiries on the currently available services.
Please confirm the availability of the equipment before making reservations; you may email us for more details.
Please check the available dates for your intended schedule of equipment use. For reservations, please accomplish Order Form III – Equipment Rental.
Training workshops on proteomic and metabolomic research. Please contact the PPMF for available and customized training modules.
Customizable services for your different protein needs.
For consultations, you may email us at [email protected] with the subject “CONSULT: [Topic/Service]”
A formal request for quotation of PPMF’s services. You may email us with the subject “RFQ: [Date MMDDYY] – [Last Name]” for other inquiries or concerns.
Please thoroughly read the sample submission requirements before sending us your samples.