Protein Purification (FPLC)

These procedures aim to purify proteins of interest from a crude protein extract. Proteins in solution (e.g. cell lysate) can be separated based on their interactions with different chromatographic columns via the ÄKTA Pure 25 L1 FPLC System.

Separate polar molecules according to their charged groups. Target charged proteins may be purified from solution using these types of columns such as sulfopropyl (SP), carboxymethyl (CM), diethylaminoethyl (DEAE), quaternary ammonium (Q)

Separate proteins using Ni Sepharose affinity resin columns that allow selective binding of proteins that have a 6X His-Tag

Separate protein mixture components based on their relative sizes. Columns of different pore sizes are used to target proteins based on their expected molecular weights.