High-Resolution Mass Spectrometry

This powerful method analyzes compounds and biomolecules by fragmenting them into ions that can be detected by the Orbitrap Fusion Tribrid high resolution mass spectrometer. Prior to mass spectrometry, sample components will be separated by reverse-phase liquid chromatography using Vanquish Horizon UHPLC for metabolite extracts or Ultimate 3000 RSLCNano nano HPLC for peptide/protein samples.

Determine the accurate monoisotopic mass of your pure protein or metabolite.

Obtain a proteomic profile of an organism in a given condition. Bottom-up and top-down proteomics can be performed depending on sample condition.

Perform high-throughput metabolite profiling of a biological sample along with putative identification via database search that could provide a comprehensive insight about the metabolic mechanisms of the specimen.

Relative quantification using reference proteome/metabolome. Observe relative changes in amounts of the peptide or metabolite among two or more varied sets of samples without the need for labeling methods.

Absolute quantification against an internal standard. This is suitable for samples that cannot be quantified using conventional means (HPLC with UV-Vis detector, etc.).

Simultaneously detect and quantify up to 10 samples using isobaric isotope tags such as the TMT in a single LC-MS run.

Compare the pure sample’s mass spectrum against libraries containing mass spectra of thousands of compounds to find the best matches for its putative identity.

Determine the putative identity of peptides from novel or unsequenced sources using de novo sequencing. The peptide will be fragmented via CID, HCD, ETD and/or EThcD.

Determine the putative identity of small molecules from novel sources using de novo sequencing. The peptide will be fragmented via CID, HCD, ETD, and/or EThcD.

Determine the protein’s possible post-translational modifications (PTMs) via software analysis of its mass spectra.