Kinetic Capillary Electrophoresis – a Swiss Army Knife for Studying Kinetics of Protein-DNA Interactions

Protein-DNA interactions play an important role in gene expression, DNA replication, DNA integrity control, and DNA damage repair. Understanding the mechanisms of these processes requires the knowledge of kinetics (rate constants) of reversible Protein-DNA binding. Electrophoresis has been the workhorse method for in vitro studies of such interactions.