Capacity building for omics analysis
The Protein, Proteomics and Metabolomics Facility (PPMF) hosts research projects, and offer services centered on the assessment of total protein expression (proteomics) as well as total metabolite populations in test samples (metabolomics). These provide the capability to assess how genomic potentials are translated to functional traits.
Further characterization of key proteins may be done with the PPMF Suite of protein analysis services. These cover applications for the production, purification, and biophysical analysis of target protein samples.
For consultations, you may contact us at ppmfacility@pgc.up.edu.ph with the subject “CONSULT: [topic/service]” or call us at 898-18500 loc 4711
These methods aim to break down cell membranes for the release and isolation of proteins and metabolites from the specimen for further purification and analysis.
These are a selection of miscellaneous processing methods of samples performed prior to purification and analysis.
These procedures aim to purify proteins of interest from a crude protein extract. Proteins in solution (e.g. cell lysate) can be separated based on their interactions with different chromatographic columns via the ÄKTA Pure 25 L1 FPLC System.
These methods are for determining the concentrations of biomolecules in samples using colorimetric methods.
These methods are comprised of different techniques such as polyacrylamide gel electrophoresis (PAGE) for molecular weight determination, western blot analysis for detection of specific molecules, surface plasmon resonance (SPR) analysis for studying the binding kinetics of molecular interactions, Circular Dichroism – Optical Rotatory Dispersion (CD-ORD) spectroscopy for secondary structure determination.
This powerful method analyzes compounds and biomolecules by fragmenting them into ions that can be detected by the Orbitrap Fusion Tribrid high resolution mass spectrometer. Prior to mass spectrometry, sample components will be separated by reverse-phase liquid chromatography using Vanquish Horizon UHPLC for metabolite extracts or Ultimate 3000 RSLCNano nano HPLC for peptide/protein samples.
Please confirm the availability of the equipment before making reservations, you may email us for more details.
A formal request for quotation of PPMF’s services. You may email us with the subject “RFQ: [Date MMDDYY] – [Last Name]” for other inquiries or concerns.
Please read and understand the terms and conditions of the facility: 1) Terms and Conditions for Service Analysis 2) Terms and Conditions for Equipment Rental 3) Terms and Conditions for Training Service
Please read the sample submission requirements thoroughly before sending us your samples. Noncompliance is subject to resubmission. Sample receiving will be from Monday to Thursday, 8:00 AM to 3:00 PM.
Customizable services for your different protein needs.
Ongoing and approved research projects of PPMF. For collaborations, you may email us for inquiries with the subject “COLLAB: [topic/field]”
Watch the compilation of webinars hosted by PPMF. For other upcoming events and updates, go to PGC’s News and Events tab.
Training workshops on proteomic and metabolomic research. Please contact the PPMF for available and customized training modules.
If you have more questions, feel free to reach out to us via ppmfacility@pgc.up.edu.ph