Cytiva (Biacore X100)
  • Affinity range: fM – mM
  • Association Rate (ka) (proteins): 103 to 107 M-1 s-1 
  • Capacity: Max. 15 samples or 15 vials
  • Concentration range: 10 pM – 2 mM
  • Flow rate: 1 – 100 uL/min
  • Immobilized Molecule Consumption: Typically, 1 ug
  • Injection volume: 5 – 90 uL
  • Molecular Weight Limit: > Mr 100
  • Number of flow cells: 2
  • Precision (concentration analysis): <5% CV
  • Sample Volume (kinetics): 32 – 120 uL
  • Sample volume min.: injection volume +20 – 30 uL (application dependent)
  • Analyte/Ligand: Wet sample in buffer solution
  • Concentration: 0.1-1 mg/mL
  • Min. Volume: 200 uL
  • Purity: High purity
  • Temperature: 4 – 40 °C
  • Known pH for ligand immobilization step and analyte binding step
  • Others: SPR chip if Client-provided, and experiment parameters to be used should be known, provide pH of buffer/solvents