Cytiva (Biacore X100)
- Affinity range: fM – mM
- Association Rate (ka) (proteins): 103 to 107 M-1 s-1
- Capacity: Max. 15 samples or 15 vials
- Concentration range: 10 pM – 2 mM
- Flow rate: 1 – 100 uL/min
- Immobilized Molecule Consumption: Typically, 1 ug
- Injection volume: 5 – 90 uL
- Molecular Weight Limit: > Mr 100
- Number of flow cells: 2
- Precision (concentration analysis): <5% CV
- Sample Volume (kinetics): 32 – 120 uL
- Sample volume min.: injection volume +20 – 30 uL (application dependent)
- Analyte/Ligand: Wet sample in buffer solution
- Concentration: 0.1-1 mg/mL
- Min. Volume: 200 uL
- Purity: High purity
- Temperature: 4 – 40 °C
- Known pH for ligand immobilization step and analyte binding step
- Others: SPR chip if Client-provided, and experiment parameters to be used should be known, provide pH of buffer/solvents
