Protein Extraction

Methods that aim on breaking down cell membranes for the release and isolation of proteins and metabolites from the specimen for further purification and analysis.

Disrupt cellular membranes by employing an ultrasonic probe.

Employ high-pressure (20,000-40,000 psi) extrusion to disrupt resilient plasma membranes and cell walls of the samples.

Disrupt cellular membranes using proteolytic enzymes, such as lysozyme.

Recover aggregated proteins from inclusion bodies with solubilizing agents or denaturants such as urea, sodium dodecyl sulfate (SDS), Triton X-100, etc.