BMG Labtech (CLARIOstar)
  • Detection modes: 
    • UV/vis absorbance
    • Fluorescence intensity (FI), incl. FRET
    • Luminescence (flash and glow) – incl. BRET
    • Fluorescence polarization (FP)
    • Time-resolved fluorescence (TRF)
    • TR-FRET
    • AlphaScreen/AlphaLISA/AlphaPlex
  • Measurement modes
    • Top and bottom reading
    • Endpoint and kinetic 
    • Sequential multi-excitation
    • Sequential multi-emission
    • Spectral scanning (fluorescence, luminescence, absorbance)
    • Ratiometric measurements
    • Well scanning
  • Microplate formats: 6 – 1536 well plates
  • Light source: Xenon flash lamp
  • Spectral range:
    • Filters:
      • FI, FP, TRF: 240 – 740 nm or 240 – 900 nm (red-shifted PMT)
      • Luminescence: 240 – 740 nm
    • LVF Monochromators:
      • FI: 320 – 740 nm or 320 – 840 nm (red-shifted PMT)
      • Luminescence: 320 – 740 nm
    • Linear Variable Dichroic
      • 340 – 740 nm or 340 – 760 nm (red-shifted PMT)
    • Spectrometer
      • Abs: 220 – 1000 nm; wavelength precision <0.5 nm
  • Wet sample in buffer solution, sample in buffer/solvent
  • Temperature: 25 °C (if not, specify temperature ranging from 25-65 °C)
  • Others: Avoid using acetone or chlorinated hydrocarbons at high concentrations (i.e., chloroform, aromatic hydrocarbons, such as toluene or benzene, etc.)