BMG Labtech (CLARIOstar)
- Detection modes:
- UV/vis absorbance
- Fluorescence intensity (FI), incl. FRET
- Luminescence (flash and glow) – incl. BRET
- Fluorescence polarization (FP)
- Time-resolved fluorescence (TRF)
- TR-FRET
- AlphaScreen/AlphaLISA/AlphaPlex
- Measurement modes
- Top and bottom reading
- Endpoint and kinetic
- Sequential multi-excitation
- Sequential multi-emission
- Spectral scanning (fluorescence, luminescence, absorbance)
- Ratiometric measurements
- Well scanning
- Microplate formats: 6 – 1536 well plates
- Light source: Xenon flash lamp
- Spectral range:
- Filters:
- FI, FP, TRF: 240 – 740 nm or 240 – 900 nm (red-shifted PMT)
- Luminescence: 240 – 740 nm
- LVF Monochromators:
- FI: 320 – 740 nm or 320 – 840 nm (red-shifted PMT)
- Luminescence: 320 – 740 nm
- Linear Variable Dichroic
- 340 – 740 nm or 340 – 760 nm (red-shifted PMT)
- Spectrometer
- Abs: 220 – 1000 nm; wavelength precision <0.5 nm
- Filters:
- Wet sample in buffer solution, sample in buffer/solvent
- Temperature: 25 °C (if not, specify temperature ranging from 25-65 °C)
- Others: Avoid using acetone or chlorinated hydrocarbons at high concentrations (i.e., chloroform, aromatic hydrocarbons, such as toluene or benzene, etc.)