[Training] Protein Analysis Workflow: Protein Purification and Characterization
Hosted by the Protein, Proteomics, and Metabolomics Facility (PPMF) in collaboration with Chemoscience and Cytiva
Date: November 6-7, 2025 | Time
Course Description:
This course aims to provide a thorough understanding of general topics such as recombinant protein expression, purification, quantification, and characterization. This workshop will also provide insight into the general pipeline for recombinant protein handling and the fundamental theories and applications involved for Fast Protein Liquid Chromatography.
Registration fee: 11,700 PHP
Limited slots are available, and registration is open until October 31, 2025. Confirmed registrants will receive details on the payment process through their registered email.
***with Twelve (12) CPD units for Chemistry
Learning Outcomes:
– Concepts behind protein purification
– Operation of the AKTA Pure 25L1 Fast Protein Liquid Chromatography System
– Principles and process behind protein detection, quantification and characterization
– Learning to do the SDS-PAGE to characterize proteins and the Bradford assay to quantify proteins
– Learning the data analysis process for protein characterization and quantification
– Learning how to present SDS-PAGE gels and process bradford assay data
Speakers & Facilitators:
Arra Asejo, MSC – is a detail‑oriented biologist and biochemist from Quezon City with an MS in Biochemistry (DOST scholar) and a BS in Biology. She currently works as a Junior Research Analyst at the Protein, Proteomics, and Metabolomics Facility, Philippine Genome Center, where she conducts protein analyses, develops SOPs, and contributes to proposals and manuscripts. Her prior roles include an MS Graduate Fellow at DOST—leading R&D initiatives, managing lab operations, and preparing technical reports—and a Biochemistry Instructor responsible for curriculum and lab protocol development. Arra excels at project coordination, scientific writing, and data interpretation, and has driven capacity‑building efforts in in‑silico protein analysis. She combines strong technical skills with experience in stakeholder communication and laboratory management to support translational research and facility operations.
John Paulo Austria, RCHT – is a Senior Research Analyst at the Protein, Proteomics, and Metabolomics Facility, Philippine Genome Center with hands‑on expertise in protein biochemistry workflows, FPLC method development, SPR and spectrophotometric analyses, and LC‑MS/MS workflows. He is a candidate for the Master of Science in Archaeology (with only thesis residency left) from the University of the Philippines Diliman and a Bachelor of Applied Science in Laboratory Technology from TUP‑Manila, and is a licensed Chemical Technician. Paulo is the current head of the protein analysis services of the PGC-PPMF, and has led method development, authored SOPs, validated client reports, and delivered hands‑on workshops while supporting proposal writing and quality systems . His earlier roles include research assistant and laboratory technician where he developed extraction and purification protocols, supported animal house operations, and facilitated HPLC trainings. He combines fieldwork experience and laboratory management with strong technical competencies in protein extraction, purification, biophysical characterization, and execution of training programs
Stephen Kyle Arcan – is a molecular biology graduate (Magna Cum Laude) and current Master’s student at UP Diliman, serving as a Junior Research Associate at the Protein, Proteomics, and Metabolomics Facility, Philippine Genome Center. He conducts end‑to‑end protein analyses, supports client consultations, and contributes to proposals, manuscripts, and training workshops. His technical strengths span protein workflows (extraction, FPLC purification, SPR), molecular biology and NGS pipelines, bioinformatics (docking, MD, variant analysis), and microbiology techniques including bacteriophage work. He has authored and co‑authored multiple publications and won presentation awards for research on quorum sensing and immune‑related protein characterization. Active in academic leadership and outreach, he pairs hands‑on lab expertise with computational skills to drive integrative research projects.
For questions, email us at:
capacitydevelopment@pgc.up.edu.ph or ppmfacility@pgc.up.edu.ph | +63-2-8981-8500 Local 4711
