Sample Preparation
These are a selection of miscellaneous processing methods of samples performed prior to purification and analysis.
Completely remove the solvent from samples through freeze drying with temperatures up to -110°C
Increase the concentration of samples by removing excess solvent via centrifugation with simultaneous reduction of pressure
Removes contaminants (e.g. salts, reducing agents, etc.) in a solution by diffusion through a semipermeable membrane, such as dialysis tubing.
Replace the buffering solvent of the sample through the use of a stirred cell membrane filter which retains solutes of particular molecular weights
- Perform reduction-alkylation and proteolytic digestion (e.g. tryptic digestion) on semi-purified and/or purified protein extracts as preparation for further analysis.
- Solid-phase extraction (SPE) treatment or sample clean-up using C18 Spin Columns on peptide or protein extracts
- Sample Concentration via speed-vac or lyophilization
- Resuspension in LC-MS grade methanol, acetonitrile, water, or any combination of the three
- Solid-phase extraction, enrichment, or cleanup using C18 or other stationary phases