From October 3 to 7, 2022, the Protein, Proteomics, and Metabolomics Facility (PPMF) research and laboratory staff of the Philippine Genome Center participated in further training on Liquid Chromatography-Mass Spectrometry (LC-MS) at the Functional Proteomics Laboratory (FPL), Institute of Molecular and Cell Biology (IMCB), Biopolis, Singapore.

 

 

 

 

 

 

 

 

 

 

 

PGC-PPMF staff (L-R) Robert Jervine Ortega and Owen Tito Mallapre

The training was conducted by Radoslaw Sobota, Ph.D., and his research team, comprised of 5-days of mass spectrometry lectures and demos on sample preparation and data processing.

 

 

 

 

Proteos Building, Biopolis, Singapore

The PGC-PPMF staff: Robert Jervine Ortega, Owen Tito Mallapre, and Abigail Ruth Velasquez were able to tour the IMCB laboratories and attended lectures on the following topics:

  • Introduction to Mass Spectrometry Proteomics
  • Sample Preparation for Mass Spectrometry-based Proteomics
  • Sample-specific Considerations for Proteomics
  • Data Processing using Proteome Discoverer
  • Skyline Demo and Online Resources

LC-MS proteomics training at Biopolis, Singapore, hosted by the Functional Proteomics Laboratory (FPL),
Institute of Molecular and Cell Biology (IMCB)

 

PPMF aims to promote the continued improvement of Omics-based research in the Philippines and to provide proteomic and metabolomic services for research projects conducted by local researchers.

This powerful method analyzes compounds and biomolecules by fragmenting them into ions that can be detected by the Orbitrap Fusion Tribrid high resolution mass spectrometer. Prior to mass spectrometry, sample components will be separated by reverse-phase liquid chromatography using Vanquish Horizon UHPLC for metabolite extracts or Ultimate 3000 RSLCNano nano HPLC for peptide/protein samples.

 

 

 

 

 

 

 

 

LC-MS/MS system that has a triple quadrupole with linear ion trap function